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Evaluation of a real-time PCR in combination with a cultivation method for the detection of Brucella abortus in organs of infected cattle in southern Italy

Authors :
Manzulli Viviana
Rondinone Valeria
Serrecchia Luigina
Petrella Antonio
Scaltrito Domenico
Marino Leonardo
Pace Lorenzo
Luigia Prencipe Maria
Cipolletta Dora
Nitti Mauro
Fasanella Antonio
Poppa Elena
Galante Domenico
Source :
Journal of Veterinary Research, Vol 66, Iss 4, Pp 559-563 (2022)
Publication Year :
2022
Publisher :
Sciendo, 2022.

Abstract

Brucellosis is a widespread zoonosis of great economic importance for livestock farming in many areas of the world. It is a highly infectious disease which is diagnosed using conventional serological and microbiological methods. The aim of this study was to assess the efficiency of a specific real-time PCR in combination with broth cultivation in detecting Brucella spp. in organs of infected cattle, in order to compare the sensitivity of the two approaches and the time needed in them until a correct diagnosis is made.

Details

Language :
English
ISSN :
24508608
Volume :
66
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Journal of Veterinary Research
Publication Type :
Academic Journal
Accession number :
edsdoj.2037edac775427dadc0516f4f7a5f42
Document Type :
article
Full Text :
https://doi.org/10.2478/jvetres-2022-0062