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Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
- Source :
- Nutrients, Vol 6, Iss 7, Pp 2668-2680 (2014)
- Publication Year :
- 2014
- Publisher :
- MDPI AG, 2014.
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Abstract
- Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound’s growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis.
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 6
- Issue :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- Nutrients
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.2072a2cc825b4fae80efa5ad54dcfc1d
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/nu6072668