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Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer

Authors :
Zhiyu Zhang
Chunhao Yu
Chun-Feng Zhang
Xiao-Hui Wu
Xiao-Dong Wen
Samantha Anderson
Wei Du
Wei-Hua Huang
Shao-Ping Li
Chong-Zhi Wang
Chun-Su Yuan
Source :
Nutrients, Vol 6, Iss 7, Pp 2668-2680 (2014)
Publication Year :
2014
Publisher :
MDPI AG, 2014.

Abstract

Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound’s growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis.

Details

Language :
English
ISSN :
20726643
Volume :
6
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Nutrients
Publication Type :
Academic Journal
Accession number :
edsdoj.2072a2cc825b4fae80efa5ad54dcfc1d
Document Type :
article
Full Text :
https://doi.org/10.3390/nu6072668