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Fungal Community Analysis and Biodeterioration of Waterlogged Wooden Lacquerware from the Nanhai No. 1 Shipwreck

Authors :
Yin Jia
Liuyu Yin
Fengyu Zhang
Mei Wang
Mingliang Sun
Cuiting Hu
Zijun Liu
Yue Chen
Jie Liu
Jiao Pan
Source :
Applied Sciences, Vol 10, Iss 11, p 3797 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

To avoid the lacquerware of the Nanhai No. 1 shipwreck from being corroded by microorganisms and to improve the knowledge on microbial ecology of the wood lacquers, we conducted a series of tests on the two water samples storing the lacquerware and colonies on the surface of the lacquerware. The high-throughput sequencing detected dominant fungal communities. After that, the fungal strains were isolated and then identified by amplification of ITS- 18S rRNA. Then the activity of ligninolytic and cellulolytic enzymes was detected on potato dextrose agar (PDA) plates with 0.04% (v/v) guaiacol and carboxymethyl cellulose (CMC) agar plates. Finally, we tested the biocide susceptibility of these fungi. Penicillium chrysogenum (NK-NH3) and Fusarium solani (NK- NH1) were the dominant fungi in the sample collected in April 2016 and June 2017. What is more, both showed activity of ligninolytic and cellulolytic enzymes. Four biocidal products (Preventol® D7, P91, BIT 20N, and Euxyl® K100) inhibited the growth of the fungal species in vitro effectively. In further research, the microbial community and environmental parameters in the museum should be monitored to assess the changes in the community and to detect potential microbial outbreaks.

Details

Language :
English
ISSN :
10113797 and 20763417
Volume :
10
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Applied Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.211c9e18e59483884d5f37ae7a35d7f
Document Type :
article
Full Text :
https://doi.org/10.3390/app10113797