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Interferon inducible pseudouridine modification in human mRNA by quantitative nanopore profiling
- Source :
- Genome Biology, Vol 22, Iss 1, Pp 1-14 (2021)
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Abstract Pseudouridine (Ψ) is an abundant mRNA modification in mammalian transcriptome, but its functions have remained elusive due to the difficulty of transcriptome-wide mapping. We develop a nanopore native RNA sequencing method for quantitative Ψ prediction (NanoPsu) that utilizes native content training, machine learning modeling, and single-read linkage analysis. Biologically, we find interferon inducible Ψ modifications in interferon-stimulated gene transcripts which are consistent with a role of Ψ in enabling efficacy of mRNA vaccines.
Details
- Language :
- English
- ISSN :
- 1474760X
- Volume :
- 22
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Genome Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.2174ae8a7ba43d59f1fde52b05e3832
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s13059-021-02557-y