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Evaluation of direct metagenomics and target enriched approaches for high-throughput sequencing of field rabies viruses
- Source :
- Journal of Veterinary Research, Vol 63, Iss 4, Pp 471-479 (2019)
- Publication Year :
- 2019
- Publisher :
- Sciendo, 2019.
-
Abstract
- High-throughput sequencing (HTS) identifies random viral fragments in environmental samples metagenomically. High reliability gains it broad application in virus evolution, host-virus interaction, and pathogenicity studies. Deep sequencing of field samples with content of host genetic material and bacteria often produces insufficient data for metagenomics and must be preceded by target enrichment. The main goal of the study was the evaluation of HTS for complete genome sequencing of field-case rabies viruses (RABVs).
- Subjects :
- rabies virus
hts
complete genome
field samples
Veterinary medicine
SF600-1100
Subjects
Details
- Language :
- English
- ISSN :
- 24508608
- Volume :
- 63
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Veterinary Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.22096ca1b94c4c60adee9fc4a63e1096
- Document Type :
- article
- Full Text :
- https://doi.org/10.2478/jvetres-2019-0067