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A sensitive biomimetic enzyme-linked immunoassay method based on Au@Pt@Au composite nanozyme label and molecularly imprinted biomimetic antibody for histamine detection
- Source :
- Food and Agricultural Immunology, Vol 32, Iss 1, Pp 592-605 (2021)
- Publication Year :
- 2021
- Publisher :
- Taylor & Francis Group, 2021.
-
Abstract
- In this paper, a sensitive biomimetic enzyme-linked immunoassay (BELISA) method based on an Au@Pt@Au composite nanozyme label was developed for histamine (HA) detection. A molecular imprinted polymer prepared directly on the 96-well plate served as a biomimetic antibody. A BELISA method was developed for HA standard solutions at concentrations ranging from 0.01 to 100.00 mg/L. Under optimal conditions, the novel BELISA method had a low limit of detection (0.069 mg/L) and high sensitivity (7.20 mg/L). The HA spiked in yellow rice wine and liqueur samples were detected by this BELISA method with the recoveries ranging from 84.28% to 108.82%. There was no significant difference of the results between the BELISA method and HPLC for the detection of HA in the red and white grape wine samples.
Details
- Language :
- English
- ISSN :
- 09540105 and 14653443
- Volume :
- 32
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Food and Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.225a770f42e44433901cc38cbbab9cd8
- Document Type :
- article
- Full Text :
- https://doi.org/10.1080/09540105.2021.1978945