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Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny

Authors :
Leonhard Linta
Marianne Stockmann
Qiong Lin
André Lechel
Christian Proepper
Tobias M. Boeckers
Alexander Kleger
Stefan Liebau
Source :
Stem Cells International, Vol 2013 (2013)
Publication Year :
2013
Publisher :
Hindawi Limited, 2013.

Abstract

Ion channels are involved in a large variety of cellular processes including stem cell differentiation. Numerous families of ion channels are present in the organism which can be distinguished by means of, for example, ion selectivity, gating mechanism, composition, or cell biological function. To characterize the distinct expression of this group of ion channels we have compared the mRNA expression levels of ion channel genes between human keratinocyte-derived induced pluripotent stem cells (hiPSCs) and their somatic cell source, keratinocytes from plucked human hair. This comparison revealed that 26% of the analyzed probes showed an upregulation of ion channels in hiPSCs while just 6% were downregulated. Additionally, iPSCs express a much higher number of ion channels compared to keratinocytes. Further, to narrow down specificity of ion channel expression in iPS cells we compared their expression patterns with differentiated progeny, namely, neurons and cardiomyocytes derived from iPS cells. To conclude, hiPSCs exhibit a very considerable and diverse ion channel expression pattern. Their detailed analysis could give an insight into their contribution to many cellular processes and even disease mechanisms.

Subjects

Subjects :
Internal medicine
RC31-1245

Details

Language :
English
ISSN :
1687966X and 16879678
Volume :
2013
Database :
Directory of Open Access Journals
Journal :
Stem Cells International
Publication Type :
Academic Journal
Accession number :
edsdoj.22af6c40ef2e4745b80ca22015e2510b
Document Type :
article
Full Text :
https://doi.org/10.1155/2013/784629