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Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals
- Source :
- Brazilian Archives of Biology and Technology, Vol 51, Iss 5, Pp 917-922 (2008)
- Publication Year :
- 2008
- Publisher :
- Instituto de Tecnologia do Paraná (Tecpar), 2008.
-
Abstract
- The purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical cancer. GBV-C/HGV RNA was detected by reverse transcriptase and polymerase chain reaction (RT-PCR). Only one woman presented co-infection with human papilloma virus (HPV). The GBV-C/HGV RNA was detected in 13/73 (17.57%) healthy women and it's prevalence in participating women between 28-43 years old was 53.85%. No association was found with GBV-C/HGV for the age of first sexual intercourse and number of pregnancies. In GBV-C/HGV RNA positive women, 69.23% were married. In conclusion, the present findings show that cervical and vaginal specimens could contain the GBV-C/HGV RNA.O objetivo do presente estudo foi avaliar a transmissão sexual de GBV-C/HBV, através da detecção do RNA viral em raspados cérvico-vaginais. Portanto, a presença do RNA GBV-C/HGV foi investigada em raspados cérvico-vaginais em mulheres aparentemente saudáveis que realizaram exames preventivos para câncer cervical. GBV-C/HGV RNA foi detectado por reação de transcriptase reversa e reação em cadeia da polimerase (RT-PCR). Apenas uma mulher apresentou a co-infecção com o papiloma vírus humano (HPV). O RNA GBV-C/HGV foi detectado em 13/73 (17,57%) mulheres saudáveis e sua prevalência entre participantes da idade de 28-43 anos foi de 53,85%. Não foi observada relação entre a presença do RNA GBV-C/HGV com a idade de primeira relação sexual, nem com o número de gestações. Entre as mulheres que apresentavam o RNA viral, 69,23% eram casadas. O presente estudo demonstrou que secreções cérvico-vaginais podem conter o RNA viral GBV-C/HBV.
- Subjects :
- GBV-C/HGV RNA
healthy women
cervicovaginal smears
Biotechnology
TP248.13-248.65
Subjects
Details
- Language :
- English
- ISSN :
- 15168913 and 16784324
- Volume :
- 51
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- Brazilian Archives of Biology and Technology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.23bd61bbfa9e499abd8a09d499e58d49
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/S1516-89132008000500007