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Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals

Authors :
Maria Angelica Ehara Watanabe
Ana Karina Melim Benthien Miquelão
Carlos Eduardo Coral de Oliveira
Karen Brajão de Oliveira
Thiago Franco Nasser
Mateus Nóbrega Aoki
Patrícia Sayuri Suzuki
Marla Karine Amarante
Emerson José Venâncio
Elbens Marcos Minoreli de Azevedo
Source :
Brazilian Archives of Biology and Technology, Vol 51, Iss 5, Pp 917-922 (2008)
Publication Year :
2008
Publisher :
Instituto de Tecnologia do Paraná (Tecpar), 2008.

Abstract

The purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical cancer. GBV-C/HGV RNA was detected by reverse transcriptase and polymerase chain reaction (RT-PCR). Only one woman presented co-infection with human papilloma virus (HPV). The GBV-C/HGV RNA was detected in 13/73 (17.57%) healthy women and it's prevalence in participating women between 28-43 years old was 53.85%. No association was found with GBV-C/HGV for the age of first sexual intercourse and number of pregnancies. In GBV-C/HGV RNA positive women, 69.23% were married. In conclusion, the present findings show that cervical and vaginal specimens could contain the GBV-C/HGV RNA.O objetivo do presente estudo foi avaliar a transmissão sexual de GBV-C/HBV, através da detecção do RNA viral em raspados cérvico-vaginais. Portanto, a presença do RNA GBV-C/HGV foi investigada em raspados cérvico-vaginais em mulheres aparentemente saudáveis que realizaram exames preventivos para câncer cervical. GBV-C/HGV RNA foi detectado por reação de transcriptase reversa e reação em cadeia da polimerase (RT-PCR). Apenas uma mulher apresentou a co-infecção com o papiloma vírus humano (HPV). O RNA GBV-C/HGV foi detectado em 13/73 (17,57%) mulheres saudáveis e sua prevalência entre participantes da idade de 28-43 anos foi de 53,85%. Não foi observada relação entre a presença do RNA GBV-C/HGV com a idade de primeira relação sexual, nem com o número de gestações. Entre as mulheres que apresentavam o RNA viral, 69,23% eram casadas. O presente estudo demonstrou que secreções cérvico-vaginais podem conter o RNA viral GBV-C/HBV.

Details

Language :
English
ISSN :
15168913 and 16784324
Volume :
51
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Brazilian Archives of Biology and Technology
Publication Type :
Academic Journal
Accession number :
edsdoj.23bd61bbfa9e499abd8a09d499e58d49
Document Type :
article
Full Text :
https://doi.org/10.1590/S1516-89132008000500007