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Structural Analysis of Target Protein by Substituted Cysteine Accessibility Method

Authors :
Tetsuo Cai
Taisuke Tomita
Source :
Bio-Protocol, Vol 8, Iss 17 (2018)
Publication Year :
2018
Publisher :
Bio-protocol LLC, 2018.

Abstract

Substituted Cysteine Accessibility Method (SCAM) is a biochemical approach to investigate the water accessibility or the spatial distance of particular cysteine residues substituted in the target protein. Protein topology and structure can be annotated by labeling with methanethiosulfonate reagents that specifically react with the cysteine residues facing the hydrophilic environment, even within the transmembrane domain. Cysteine crosslinking experiments provide us with information about the distance between two cysteine residues. The combination of these methods enables us to obtain information about the structural changes of the target protein. Here, we describe the detailed protocol for structural analysis using SCAM.

Subjects

Subjects :
Biology (General)
QH301-705.5

Details

Language :
English
ISSN :
23318325
Volume :
8
Issue :
17
Database :
Directory of Open Access Journals
Journal :
Bio-Protocol
Publication Type :
Academic Journal
Accession number :
edsdoj.23c47a06d16408da063f154fcd72091
Document Type :
article
Full Text :
https://doi.org/10.21769/BioProtoc.2470