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Secretome profiling reveals acute changes in oxidative stress, brain homeostasis, and coagulation following short-duration spaceflight

Authors :
Nadia Houerbi
JangKeun Kim
Eliah G. Overbey
Richa Batra
Annalise Schweickart
Laura Patras
Serena Lucotti
Krista A. Ryon
Deena Najjar
Cem Meydan
Namita Damle
Christopher Chin
S. Anand Narayanan
Joseph W. Guarnieri
Gabrielle Widjaja
Afshin Beheshti
Gabriel Tobias
Fanny Vatter
Jeremy Wain Hirschberg
Ashley Kleinman
Evan E. Afshin
Matthew MacKay
Qiuying Chen
Dawson Miller
Aaron S. Gajadhar
Lucy Williamson
Purvi Tandel
Qiu Yang
Jessica Chu
Ryan Benz
Asim Siddiqui
Daniel Hornburg
Steven Gross
Bader Shirah
Jan Krumsiek
Jaime Mateus
Xiao Mao
Irina Matei
Christopher E. Mason
Source :
Nature Communications, Vol 15, Iss 1, Pp 1-17 (2024)
Publication Year :
2024
Publisher :
Nature Portfolio, 2024.

Abstract

Abstract As spaceflight becomes more common with commercial crews, blood-based measures of crew health can guide both astronaut biomedicine and countermeasures. By profiling plasma proteins, metabolites, and extracellular vesicles/particles (EVPs) from the SpaceX Inspiration4 crew, we generated “spaceflight secretome profiles,” which showed significant differences in coagulation, oxidative stress, and brain-enriched proteins. While >93% of differentially abundant proteins (DAPs) in vesicles and metabolites recovered within six months, the majority (73%) of plasma DAPs were still perturbed post-flight. Moreover, these proteomic alterations correlated better with peripheral blood mononuclear cells than whole blood, suggesting that immune cells contribute more DAPs than erythrocytes. Finally, to discern possible mechanisms leading to brain-enriched protein detection and blood-brain barrier (BBB) disruption, we examined protein changes in dissected brains of spaceflight mice, which showed increases in PECAM-1, a marker of BBB integrity. These data highlight how even short-duration spaceflight can disrupt human and murine physiology and identify spaceflight biomarkers that can guide countermeasure development.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
15
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.247c896f001a435c9ecc55bb10c04051
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-024-48841-w