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Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil

Authors :
Roozbeh Tahmasebi
Antonio Charlys da Costa
Kaelan Tardy
Rory J. Tinker
Flavio Augusto de Padua Milagres
Rafael Brustulin
Maria da Aparecida Rodrigues Teles
Rogério Togisaki das Chagas
Cassia Vitória de Deus Alves Soares
Aripuana Sakurada Aranha Watanabe
Cecilia Salete Alencar
Fabiola Villanova
Xutao Deng
Eric Delwart
Adriana Luchs
Élcio Leal
Ester Cerdeira Sabino
Source :
Viruses, Vol 12, Iss 5, p 508 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Human Adenovirus species C (HAdV-C) is the most common etiologic agent of respiratory disease. In the present study, we characterized the nearly full-length genome of one potential new HAdV-C recombinant strain constituted by Penton and Fiber proteins belonging to type 89 and a chimeric Hexon protein of types 1 and 89. By using viral metagenomics techniques, we screened out, in the states of Tocantins and Pará, Northern and North regions of Brazil, from 2010 to 2016, 251 fecal samples of children between 0.5 to 2.5 years old. These children were presenting acute diarrhea not associated with common pathogens (i.e., rotavirus, norovirus). We identified two HAdV-C strains in two distinct patients. Phylogenetic analysis performed using all complete genomes available at GenBank database indicated that one strain (HAdV-C BR-245) belonged to type 1. The phylogenetic analysis also indicated that the second strain (HAdV-C BR-211) was located at the base of the clade formed by the newly HAdV-C strains type 89. Recombination analysis revealed that strain HAdV-C BR-211 is a chimera in which the variable regions of Hexon gene combined HAdV-C1 and HAdV-C89 sequences. Therefore, HAdV-C BR-211 strain possesses a genomic backbone of type HAdV-C89 and a unique insertion of HAdV-C1 in the Hexon sequence. Recombination may play an important driving force in HAdV-C diversity and evolution. Studies employing complete genomic sequencing on circulating HAdV-C strains in Brazil are needed to understand the clinical significance of the presented data.

Details

Language :
English
ISSN :
19994915
Volume :
12
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.25fe59d9e2ae4971b308f4bfcc2997de
Document Type :
article
Full Text :
https://doi.org/10.3390/v12050508