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Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition

Authors :
ZHANG Huazhi
LI Xiaoshuang
GUO Xiaoye
ZHAO Mingchi
Source :
Zhongliu Fangzhi Yanjiu, Vol 46, Iss 5, Pp 401-405 (2019)
Publication Year :
2019
Publisher :
Magazine House of Cancer Research on Prevention and Treatment, 2019.

Abstract

Objective To investigate the antitumor effect of apatinib on breast cancer cells in vitro and its mechanism. Methods CCK-8 assay was used to detect the inhibitory effect of apatinib on the proliferation of breast cancer cell line MDA-MB-231. Annexin V-FITC/PI apoptosis detection kit was used to detect the effect of apatinib on the apoptosis of MDA-MB-231 cells. The effect of apatinib on lactate production in MDA-MB-231 cells was detected by lactate content assay kit. The effect of apatinib on the extracellular acidification rate of MDA-MB-231 cells was detected by glycolysis stress test kit. Results Apatinib inhibited the proliferation of breast cancer MDA-MB-231 cells in vitro in a concentration-dependent manner (P < 0.05). The half maximal inhibitory concentration (IC50) of apatinib in MDA-MB-231 cells was 1.56μmol/L for 48h treatment. Apatinib also induced the apoptosis of MDA-MB-231 cells in a concentration-dependent manner (P < 0.05). Apatinib inhibited intracellular lactate content in MDA-MB-231 cells in a concentration-dependent manner (P < 0.05). Apatinib also decreased extracellular acidification rate of MDA-MB-231 cells, including glycolytic capacity and glycolytic reserve (P < 0.05). Conclusion Apatinib could induce the apoptosis of breast cancer cell line MDA-MB-231 by inhibiting aerobic glycolysis.

Details

Language :
Chinese
ISSN :
10008578
Volume :
46
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Zhongliu Fangzhi Yanjiu
Publication Type :
Academic Journal
Accession number :
edsdoj.27b54ee84f46bf93bb18c92c447715
Document Type :
article
Full Text :
https://doi.org/10.3971/j.issn.1000-8578.2019.18.1422