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Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing

Authors :
Laura Frigotto
Matthew E. Smith
Christopher Brankin
Ashni Sedani
Simon E. Cooper
Nisha Kanwar
Daniel Evans
Stanislava Svobodova
Claudia Baar
Jacob Glanville
Christopher G. Ullman
Anna V. Hine
Source :
Antibodies, Vol 4, Iss 2, Pp 88-102 (2015)
Publication Year :
2015
Publisher :
MDPI AG, 2015.

Abstract

We have previously described ProxiMAX, a technology that enables the fabrication of precise, combinatorial gene libraries via codon-by-codon saturation mutagenesis. ProxiMAX was originally performed using manual, enzymatic transfer of codons via blunt-end ligation. Here we present Colibra™: an automated, proprietary version of ProxiMAX used specifically for antibody library generation, in which double-codon hexamers are transferred during the saturation cycling process. The reduction in process complexity, resulting library quality and an unprecedented saturation of up to 24 contiguous codons are described. Utility of the method is demonstrated via fabrication of complementarity determining regions (CDR) in antibody fragment libraries and next generation sequencing (NGS) analysis of their quality and diversity.

Details

Language :
English
ISSN :
20734468
Volume :
4
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Antibodies
Publication Type :
Academic Journal
Accession number :
edsdoj.2821e76be3f340418b074a776924229f
Document Type :
article
Full Text :
https://doi.org/10.3390/antib4020088