Back to Search
Start Over
Comprehensive integration of single-cell transcriptomic data illuminates the regulatory network architecture of plant cell fate specification
- Source :
- Plant Diversity, Vol 46, Iss 3, Pp 372-385 (2024)
- Publication Year :
- 2024
- Publisher :
- KeAi Communications Co., Ltd., 2024.
-
Abstract
- Plant morphogenesis relies on precise gene expression programs at the proper time and position which is orchestrated by transcription factors (TFs) in intricate regulatory networks in a cell-type specific manner. Here we introduced a comprehensive single-cell transcriptomic atlas of Arabidopsis seedlings. This atlas is the result of meticulous integration of 63 previously published scRNA-seq datasets, addressing batch effects and conserving biological variance. This integration spans a broad spectrum of tissues, including both below- and above-ground parts. Utilizing a rigorous approach for cell type annotation, we identified 47 distinct cell types or states, largely expanding our current view of plant cell compositions. We systematically constructed cell-type specific gene regulatory networks and uncovered key regulators that act in a coordinated manner to control cell-type specific gene expression. Taken together, our study not only offers extensive plant cell atlas exploration that serves as a valuable resource, but also provides molecular insights into gene-regulatory programs that varies from different cell types.
Details
- Language :
- English
- ISSN :
- 24682659
- Volume :
- 46
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Plant Diversity
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.28da028d00a44f8783f15994d35f8545
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.pld.2024.03.008