Back to Search Start Over

Differential Transcriptomic Signatures of Small Airway Cell Cultures Derived from IPF and COVID-19-Induced Exacerbation of Interstitial Lung Disease

Authors :
Katie Uhl
Shreya Paithankar
Dmitry Leshchiner
Tara E. Jager
Mohamed Abdelgied
Bhavna Dixit
Raya Marashdeh
Dewen Luo-Li
Kaylie Tripp
Angela M. Peraino
Maximiliano Tamae Kakazu
Cameron Lawson
Dave W. Chesla
Ningzhi Luo-Li
Edward T. Murphy
Jeremy Prokop
Bin Chen
Reda E. Girgis
Xiaopeng Li
Source :
Cells, Vol 12, Iss 20, p 2501 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Idiopathic pulmonary fibrosis (IPF) is a pathological condition wherein lung injury precipitates the deposition of scar tissue, ultimately leading to a decline in pulmonary function. Existing research indicates a notable exacerbation in the clinical prognosis of IPF patients following infection with COVID-19. This investigation employed bulk RNA-sequencing methodologies to describe the transcriptomic profiles of small airway cell cultures derived from IPF and post-COVID fibrosis patients. Differential gene expression analysis unveiled heightened activation of pathways associated with microtubule assembly and interferon signaling in IPF cell cultures. Conversely, post-COVID fibrosis cell cultures exhibited distinctive characteristics, including the upregulation of pathways linked to extracellular matrix remodeling, immune system response, and TGF-β1 signaling. Notably, BMP signaling levels were elevated in cell cultures derived from IPF patients compared to non-IPF control and post-COVID fibrosis samples. These findings underscore the molecular distinctions between IPF and post-COVID fibrosis, particularly in the context of signaling pathways associated with each condition. A better understanding of the underlying molecular mechanisms holds the promise of identifying potential therapeutic targets for future interventions in these diseases.

Details

Language :
English
ISSN :
20734409
Volume :
12
Issue :
20
Database :
Directory of Open Access Journals
Journal :
Cells
Publication Type :
Academic Journal
Accession number :
edsdoj.28fa01fc1ab7447c8f380a819c22d5c8
Document Type :
article
Full Text :
https://doi.org/10.3390/cells12202501