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Detection of African swine fever virus antibodies in serum using a pB602L protein-based indirect ELISA

Authors :
Yang Yang
Qiqi Xia
Qin Sun
Yan Zhang
Yuhao Li
Xiaochun Ma
Zhixin Guan
Junjie Zhang
Zongjie Li
Ke Liu
Beibei Li
Donghua Shao
Yafeng Qiu
Zhiyong Ma
Jianchao Wei
Source :
Frontiers in Veterinary Science, Vol 9 (2022)
Publication Year :
2022
Publisher :
Frontiers Media S.A., 2022.

Abstract

African Swine Fever (ASF) is an acute, highly contagious and deadly infectious disease that has a huge impact on the swine industry. It is caused by the African swine fever virus (ASFV). The most acute forms of ASF in domestic pigs have mortality rates of up to 100%. The lack of a commercial vaccine and effective therapeutic drugs has brought great challenges to the prevention and control of ASF. Current, the African swine fever virus requires a huge amount of detection, so there is a need for more sensitive and accurate detection technology. The protein pB602L, as a late non-structural protein, has a high corresponding antibody titer and strong antigenicity in infected swine. In this research, the B602L gene was constructed into the pColdI prokaryotic expression vector, and prokaryotic expression of the soluble pB602L protein was induced by IPTG. Western blot analysis demonstrated that the protein had strong immunogenicity. We established an indirect ELISA method for the detection of anti-ASFV using purified recombinant pB602L protein as antigen. The detection method showed excellent specificity without cross-reactions with antibodies against PRRSV, CSFV, JEV, and GETV. The method could detect anti-ASFV in serum samples that were diluted up to 6,400 times, showing high sensitivity. The coefficients of variation of the intra-assay and inter-assay were both

Details

Language :
English
ISSN :
22971769
Volume :
9
Database :
Directory of Open Access Journals
Journal :
Frontiers in Veterinary Science
Publication Type :
Academic Journal
Accession number :
edsdoj.291218783fe548b5be9b42cb343a7a73
Document Type :
article
Full Text :
https://doi.org/10.3389/fvets.2022.971841