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Canagliflozin attenuates the progression of atherosclerosis and inflammation process in APOE knockout mice
- Source :
- Cardiovascular Diabetology, Vol 17, Iss 1, Pp 1-12 (2018)
- Publication Year :
- 2018
- Publisher :
- BMC, 2018.
-
Abstract
- Abstract Background Sodium glucose co-transporter2 inhibitors reduce the incidence of cardiovascular events in patients with type 2 diabetes mellitus based on the results of recent cardiovascular outcome studies. Herein, we investigated the effects of long-term treatment with canagliflozin on biochemical and immunohistochemical markers related to atherosclerosis and atherosclerosis development in the aorta of apolipoprotein E knockout (Apo-E(−/−)) mice. Methods At the age of 5 weeks, mice were switched from normal to a high-fat diet. After 5 weeks, Apo-E(−/−) mice were divided into control-group (6 mice) treated with 0.5% hydroxypropyl methylcellulose and Cana-group (7 mice) treated with canagliflozin (10 mg/kg per day) per os. After 5 weeks of intervention, animals were sacrificed, and heart and aorta were removed. Sections stained with hematoxylin–eosin (H&E) were used for histomorphometry whereas Masson’s stained tissues were used to quantify the collagen content. Immunohistochemistry to assess MCP-1, CD68, a-smooth muscle actin, MMP-2, MMP-9, TIMP-1 and TIMP-2 expression was carried out and q-PCR experiments were performed to quantify mRNA expression. Results Canagliflozin-group mice had lower total-cholesterol, triglycerides and glucose levels (P
Details
- Language :
- English
- ISSN :
- 14752840
- Volume :
- 17
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Cardiovascular Diabetology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.29be01d1c57c4de18eac7a2c4b1661db
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s12933-018-0749-1