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Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus

Authors :
Mengxiang Wang
Jinxing Song
Junru Sun
Yongkun Du
Xiaodong Qin
Lu Xia
Yanan Wu
Gaiping Zhang
Source :
Viruses, Vol 14, Iss 10, p 2170 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

African swine fever (ASF), the highly lethal swine infectious disease caused by the African swine fever virus (ASFV), is a great threat to the swine industry. There is no effective vaccine or diagnostic method to prevent and control this disease currently. The p30 protein of ASFV is an important target for serological diagnosis, expressed in the early stage of viral replication and has high immunogenicity and sequence conservatism. Here, the CP204L gene was cloned into the expression vector pET-30a (+), and the soluble p30 protein was successfully expressed in the E. coli prokaryotic expression system and then labeled with horseradish peroxidase (HRP) to be the enzyme-labeled antigen. Using the purified recombinant p30 protein, a double-antigen sandwich ELISA for ASFV antibody detection was developed. This method exhibits excellent specificity, sensitivity and reproducibility in clinical sample detection with lower cost and shorter production cycles. Taken together, this study provides technical support for antibody detection for ASFV.

Details

Language :
English
ISSN :
19994915
Volume :
14
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.29c7262a6fd748b99239c33187775096
Document Type :
article
Full Text :
https://doi.org/10.3390/v14102170