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An Optimized Approach for Serial Crystallography Using Chips

Authors :
Marina Galchenkova
Aida Rahmani Mashhour
Patrick Y. A. Reinke
Sebastian Günther
Jan Meyer
Henry N. Chapman
Oleksandr M. Yefanov
Source :
Crystals, Vol 13, Iss 8, p 1225 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Serial crystallography is a rapidly developing method for the determination of the structure of biomolecules at room temperature at near-atomic resolution from an ensemble of small crystals. Numerous advances in detectors, data analysis pipelines, sample delivery methods, and crystallization protocols expand the scope of structural biology to understand the fundamental processes that take place in living cells. Many experimental strategies for serial crystallography are in use, depending on the type and sizes of the crystals or other needs of the experiment. Such strategies should ideally minimize the wastage of samples or beamtime without compromising experimental goals. This paper proposes a way to optimize beamtime utilization in serial crystallography experiments that use fixed-target sample delivery methods, such as chips. The strategy involves two key steps. Firstly, a fast raster scan of the chip is performed to determine the positions of the crystals based on their diffraction. Subsequently, a rotational series is collected at each identified crystal position, covering a narrow range of chip orientations. This approach enables the exclusion of empty positions during data acquisition, resulting in significant savings in beam time utilization and a reduced volume of measured data.

Details

Language :
English
ISSN :
13081225 and 20734352
Volume :
13
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Crystals
Publication Type :
Academic Journal
Accession number :
edsdoj.2b30a77376ea4aebb957d461e536d4df
Document Type :
article
Full Text :
https://doi.org/10.3390/cryst13081225