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Virus-induced gene silencing in the perennial woody Paeonia ostii
- Source :
- PeerJ, Vol 7, p e7001 (2019)
- Publication Year :
- 2019
- Publisher :
- PeerJ Inc., 2019.
-
Abstract
- Tree peony is a perennial deciduous shrub with great ornamental and medicinal value. A limitation of its current functional genomic research is the lack of effective molecular genetic tools. Here, the first application of a Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) in the tree peony species Paeonia ostii is presented. Two different approaches, leaf syringe-infiltration and seedling vacuum-infiltration, were utilized for Agrobacterium-mediated inoculation. The vacuum-infiltration was shown to result in a more complete Agrobacterium penetration than syringe-infiltration, and thereby determined as an appropriate inoculation method. The silencing of reporter gene PoPDS encoding phytoene desaturase was achieved in TRV-PoPDS-infected triennial tree peony plantlets, with a typical photobleaching phenotype shown in uppermost newly-sprouted leaves. The endogenous PoPDS transcripts were remarkably down-regulated in VIGS photobleached leaves. Moreover, the green fluorescent protein (GFP) fluorescence was detected in leaves and roots of plants inoculated with TRV-GFP, suggesting the capability of TRV to silence genes in various tissues. Taken together, the data demonstrated that the TRV-based VIGS technique could be adapted for high-throughput functional characterization of genes in tree peony.
Details
- Language :
- English
- ISSN :
- 21678359
- Volume :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- PeerJ
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.2dfe9343874c479d8e0690533d5508ad
- Document Type :
- article
- Full Text :
- https://doi.org/10.7717/peerj.7001