Back to Search
Start Over
MicroRNA-199a Inhibits Cellular Autophagy and Downregulates IFN-β Expression by Targeting TBK1 in Mycobacterium bovis Infected Cells
- Source :
- Frontiers in Cellular and Infection Microbiology, Vol 8 (2018)
- Publication Year :
- 2018
- Publisher :
- Frontiers Media S.A., 2018.
-
Abstract
- The mechanism by which microRNAs (miRNAs) modulate innate immunity and autophagy has not been fully elucidated in Mycobacterium bovis (M. bovis) infections. In this study, we identified that miR-199a inhibited key innate immune responses and autophagy in murine macrophages infected with M. bovis. Using ex vivo and in vitro approaches we show that the expression of miR-199a was significantly increased during M. bovis infection. Furthermore, miR-199a suppressed autophagy and interferon-β (IFN-β) production by directly targeting TANK-binding kinase 1 (TBK1) mRNA in both J774a.1 and BMDM cells. Upregulation of miR-199a or TBK1 silencing (siTBK1) inhibited maturation of autophagosomes and increased M. bovis survival. Our results demonstrate that, by targeting of TBK1, miR-199a modulates innate immune responses and promote the intracellular survival and growth of M. bovis.
Details
- Language :
- English
- ISSN :
- 22352988
- Volume :
- 8
- Database :
- Directory of Open Access Journals
- Journal :
- Frontiers in Cellular and Infection Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.2ebed22a7df04079b89d47b8e0be96c9
- Document Type :
- article
- Full Text :
- https://doi.org/10.3389/fcimb.2018.00238