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Impact of Environmental Conditions on the Protein Content of Staphylococcus aureus and Its Derived Extracellular Vesicles

Authors :
Brenda Silva Rosa da Luz
Vinícius de Rezende Rodovalho
Aurélie Nicolas
Svetlana Chabelskaya
Julien Jardin
Valérie Briard-Bion
Yves Le Loir
Vasco Ariston de Carvalho Azevedo
Éric Guédon
Source :
Microorganisms, Vol 10, Iss 9, p 1808 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Staphylococcus aureus, a major opportunistic pathogen in humans, produces extracellular vesicles (EVs) that are involved in cellular communication, the delivery of virulence factors, and modulation of the host immune system response. However, to date, the impact of culture conditions on the physicochemical and functional properties of S. aureus EVs is still largely unexplored. Here, we use a proteomic approach to provide a complete protein characterization of S. aureus HG003, a NCTC8325 derivative strain and its derived EVs under four growth conditions: early- and late-stationary growth phases, and in the absence and presence of a sub-inhibitory concentration of vancomycin. The HG003 EV protein composition in terms of subcellular localization, COG and KEGG categories, as well as their relative abundance are modulated by the environment and differs from that of whole-cell (WC). Moreover, the environmental conditions that were tested had a more pronounced impact on the EV protein composition when compared to the WC, supporting the existence of mechanisms for the selective packing of EV cargo. This study provides the first general picture of the impact of different growth conditions in the proteome of S. aureus EVs and its producing-cells and paves the way for future studies to understand better S. aureus EV production, composition, and roles.

Details

Language :
English
ISSN :
20762607
Volume :
10
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.2faed0e6004742499480aea36f6d2567
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms10091808