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Expression and distribution of the glucocorticoid receptor DlGR1 in the teleost Dicentrarchus labrax brain
- Source :
- Italian Journal of Animal Science, Vol 8, Iss 2s, Pp 854-856 (2010)
- Publication Year :
- 2010
- Publisher :
- Taylor & Francis Group, 2010.
-
Abstract
- Cortisol is the main corticosteroid secreted by the interrenal cells of the head kidney and it exerts a role in mantaining the omeostatic status in fish. In teleosts its effects are mediated through intracellular receptors expressed in several tissues, that are ligand-dependent transcription factors by binding to specific tissue DNA sequences. In Dicentrarchus labrax we previously cloned and sequenced a glucocorticoid receptor, DlGR1, isolated from leukocytes of peritoneal cavity. In this work we showed mRNA expression and tissue immunohistochemical localization of brain DlGR1 by in situ hybridization assays, with a riboprobe with DlGR1 cDNA trascriptional activation domain, and by immunohistochemical methods, using a specific antibody for a selected sequence of the receptor tran- scriptional domain. The mRNA and the protein are expressed in pyramidal cells of the optic lobe and in the small globular neurons of the diencephalon.
Details
- Language :
- English
- ISSN :
- 15944077 and 1828051X
- Volume :
- 8
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Italian Journal of Animal Science
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.305458a21c3544f690d8f19a9166720c
- Document Type :
- article
- Full Text :
- https://doi.org/10.4081/ijas.2009.s2.854