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Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography
- Source :
- Molecules, Vol 17, Iss 7, Pp 8276-8284 (2012)
- Publication Year :
- 2012
- Publisher :
- MDPI AG, 2012.
-
Abstract
- Following a constituent enrichment step on a silica gel column, four phenyl-ethanoid glycosides were successfully isolated from Cistanches deserticola and purified by preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (40:6:6:50, v/v/v/v). A total of 30.9 mg acteoside, 13.0 mg isoacteoside, 12.5 mg syringalide A 3'-α-L-rhamnopyranoside and 7.2 mg 2'-acetylacteoside with purity of higher than 95%, as determined by HPLC-ELSD, were obtained in one-step separation from 297 mg of Cistanche deserticola extract, respectively. Their structures were identified by HR-MS, 1H-NMR and 13C-NMR.
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 17
- Issue :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- Molecules
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.312d46487ae042caba7d50d5d7c45b73
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/molecules17078276