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Corn gluten meal peptides inhibit prolyl oligopeptidase and modulate α-synuclein aggregation in KCl-treated SH-SY5Y cells
- Source :
- Journal of Functional Foods, Vol 104, Iss , Pp 105501- (2023)
- Publication Year :
- 2023
- Publisher :
- Elsevier, 2023.
-
Abstract
- Prolyl oligopeptidase (POP) is known to be related to neurocognitive disorder as it degrades neuroactive peptides. POP inhibitory peptides from corn gluten meal hydrolysate (CGM-H) were identified and characterized. After a series of chromatographic separations, ALLTLSPLGPA was identified as the most effective POP inhibitor, with an IC50 value of 0.79 ± 0.004 mM. Its in silico gastrointestinal (GI)-digested peptide, SPLGPA, exhibited 3-fold lower POP inhibitory activity, while higher inhibition was observed in SH-SY5Y cells when compared to its parent peptide. In addition, 25 µM ALLTLSPLGPA notably reduced α-synuclein aggregation in KCl-treated SH-SY5Y cells. ALLTLSPLGPA and SPLGPA were characterized as mixed-type and uncompetitive inhibitors, respectively. They bound to POP via hydrogen bonds at the β-propeller domain. These results demonstrated that peptides derived from CGM could have potential for developing nutraceutical products targeting brain health.
Details
- Language :
- English
- ISSN :
- 17564646
- Volume :
- 104
- Issue :
- 105501-
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Functional Foods
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.335c78c582e481aaffd6de740d11619
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.jff.2023.105501