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A cell-type-specific atlas of the inner ear transcriptional response to acoustic trauma

Authors :
Beatrice Milon
Eldad D. Shulman
Kathy S. So
Christopher R. Cederroth
Erika L. Lipford
Michal Sperber
Jonathan B. Sellon
Heela Sarlus
Gabriela Pregernig
Benjamin Shuster
Yang Song
Sunayana Mitra
Joshua Orvis
Zachary Margulies
Yoko Ogawa
Christopher Shults
Didier A. Depireux
Adam T. Palermo
Barbara Canlon
Joe Burns
Ran Elkon
Ronna Hertzano
Source :
Cell Reports, Vol 36, Iss 13, Pp 109758- (2021)
Publication Year :
2021
Publisher :
Elsevier, 2021.

Abstract

Summary: Noise-induced hearing loss (NIHL) results from a complex interplay of damage to the sensory cells of the inner ear, dysfunction of its lateral wall, axonal retraction of type 1C spiral ganglion neurons, and activation of the immune response. We use RiboTag and single-cell RNA sequencing to survey the cell-type-specific molecular landscape of the mouse inner ear before and after noise trauma. We identify induction of the transcription factors STAT3 and IRF7 and immune-related genes across all cell-types. Yet, cell-type-specific transcriptomic changes dominate the response. The ATF3/ATF4 stress-response pathway is robustly induced in the type 1A noise-resilient neurons, potassium transport genes are downregulated in the lateral wall, mRNA metabolism genes are downregulated in outer hair cells, and deafness-associated genes are downregulated in most cell types. This transcriptomic resource is available via the Gene Expression Analysis Resource (gEAR; https://umgear.org/NIHL) and provides a blueprint for the rational development of drugs to prevent and treat NIHL.

Details

Language :
English
ISSN :
22111247
Volume :
36
Issue :
13
Database :
Directory of Open Access Journals
Journal :
Cell Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.339f5e676bfd45d480fda7df6c6888d0
Document Type :
article
Full Text :
https://doi.org/10.1016/j.celrep.2021.109758