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Differential Expression of Gene Profiles in MRGX-treated Lung Cancer
- Source :
- Journal of Pharmacopuncture, Vol 16, Iss 3, Pp 30-38 (2013)
- Publication Year :
- 2013
- Publisher :
- Korean Pharmacopuncture Institute, 2013.
-
Abstract
- Objectives: Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. Methods: To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network. Results: One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Conclusions: Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.
Details
- Language :
- English, Korean
- ISSN :
- 20936966 and 22346856
- Volume :
- 16
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Pharmacopuncture
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.33cd0f6a6bc4435b92f73a7e3191ef73
- Document Type :
- article
- Full Text :
- https://doi.org/10.3831/KPI.2013.16.018