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Effect of nicotine on biofilm formation of Streptococcus mutans isolates from smoking and non-smoking subjects

Authors :
Nasreen F. El-Ezmerli
Richard L. Gregory
Source :
Journal of Oral Microbiology, Vol 11, Iss 1 (2019)
Publication Year :
2019
Publisher :
Taylor & Francis Group, 2019.

Abstract

Objectives: To investigate effects of nicotine on biofilm formation of Streptococcus mutans isolates from oral washes of smoker and non-smoker human subjects. Materials and methods: This study was conducted using 60 S. mutans isolates with three S. mutans isolates collected from oral washes of ten smoking subjects and ten from non-smoking subjects. Biofilm was formed by culturing each S. mutans strain (10 μl) in 190 μl of TSB supplemented with 1% sucrose (TSBS) containing 0, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0, and 32.0 mg/ml of nicotine for 24 h in 5% CO2 at 37°C in 96 well microtiter plates. The absorbance values of biofilm were measured at 490 nm in a microplate spectrophotometer. Results: There was a significant effect (p-value < 0.05) of nicotine concentrations and smoking on the growth of biofilm, planktonic cells, and total absorbance, for all strains of S. mutans. Isolates from smokers had significantly more biofilm at 0–16 mg/ml of nicotine compared to those from non-smokers (p-value < 0.0001). Conclusion: S. mutans smoker isolates are more affected by high nicotine concentrations than non-smoker isolates. Clinical Relevance: The use of nicotine products increases the growth of S. mutans and may place tobacco users at risk for dental decay.

Details

Language :
English
ISSN :
20002297
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Journal of Oral Microbiology
Publication Type :
Academic Journal
Accession number :
edsdoj.342ddf8a16db4b8895c1e98b89e82abc
Document Type :
article
Full Text :
https://doi.org/10.1080/20002297.2019.1662275