Back to Search Start Over

De‐nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus

Authors :
Hong Hu
Wenhui He
Zhiguang Qu
Xiang Dong
Zhiyong Ren
Mengyuan Qin
Hao Liu
Lu Zheng
Junbin Huang
Xiao‐Lin Chen
Source :
Advanced Science, Vol 11, Iss 26, Pp n/a-n/a (2024)
Publication Year :
2024
Publisher :
Wiley, 2024.

Abstract

Abstract As a signaling molecule, nitric oxide (NO) regulates the development and stress response in different organisms. The major biological activity of NO is protein S‐nitrosylation, whose function in fungi remains largely unclear. Here, it is found in the rice blast fungus Magnaporthe oryzae, de‐nitrosylation process is essential for functional appressorium formation during infection. Nitrosative stress caused by excessive accumulation of NO is harmful for fungal infection. While the S‐nitrosoglutathione reductase GSNOR‐mediated de‐nitrosylation removes excess NO toxicity during appressorium formation to promote infection. Through an indoTMT switch labeling proteomics technique, 741 S‐nitrosylation sites in 483 proteins are identified. Key appressorial proteins, such as Mgb1, MagB, Sps1, Cdc42, and septins, are activated by GSNOR through de‐nitrosylation. Removing S‐nitrosylation sites of above proteins is essential for proper protein structure and appressorial function. Therefore, GSNOR‐mediated de‐nitrosylation is an essential regulator for appressorium formation. It is also shown that breaking NO homeostasis by NO donors, NO scavengers, as well as chemical inhibitor of GSNOR, shall be effective methods for fungal disease control.

Details

Language :
English
ISSN :
21983844
Volume :
11
Issue :
26
Database :
Directory of Open Access Journals
Journal :
Advanced Science
Publication Type :
Academic Journal
Accession number :
edsdoj.353ea2d4d34825bb868a6039c61525
Document Type :
article
Full Text :
https://doi.org/10.1002/advs.202403894