Back to Search Start Over

Single molecule characterization of individual extracellular vesicles from pancreatic cancer

Single molecule characterization of individual extracellular vesicles from pancreatic cancer

Authors :
Kathleen M. Lennon
Devin L. Wakefield
Adam L. Maddox
Matthew S. Brehove
Ari N. Willner
Krystine Garcia-Mansfield
Bessie Meechoovet
Rebecca Reiman
Elizabeth Hutchins
Marcia M. Miller
Ajay Goel
Patrick Pirrotte
Kendall Van Keuren-Jensen
Tijana Jovanovic-Talisman
Source :
Journal of Extracellular Vesicles, Vol 8, Iss 1 (2019)
Publication Year :
2019
Publisher :
Wiley, 2019.

Abstract

Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. Abbreviations: EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.

Details

Language :
English
ISSN :
20013078
Volume :
8
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Journal of Extracellular Vesicles
Publication Type :
Academic Journal
Accession number :
edsdoj.364c6031b13846c3a8651fb570b8f5c3
Document Type :
article
Full Text :
https://doi.org/10.1080/20013078.2019.1685634