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Tat-P combined with GAPR1 releases Beclin1 to promote autophagy and improve Bronchopulmonary dysplasia model

Authors :
Yahui Zhou
Yuting Zhu
Weilai Jin
Ru Yan
Yuanyuan Fang
Fan Zhang
Tonghui Tang
Si Chen
Jing Chen
Zhangbin Yu
Le Zang
Zhiwei Yu
Source :
iScience, Vol 26, Iss 9, Pp 107509- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Summary: Long-term exposure to hyperoxia can leading to the bronchopulmonary dysplasia (BPD). The progression of BPD is primarily driven by the apoptosis of alveolar epithelial cells, and the regulation of autophagy has an impact on apoptosis. This study aims to investigate the therapeutic potential and underlying mechanism of an autophagy-promoting peptide (Tat-P) in ameliorating BPD. In vitro experiments demonstrated that Tat-P promoted autophagy and partially prevented apoptosis caused by exposure to hyperoxia. Further investigation into the mechanism revealed that Tat-P competitively binds to GAPR1, displacing the Beclin1 protein and thereby inhibiting the apoptosis. In vivo experiments conducted on Sprague-Dawley pups exposed to high oxygen levels demonstrated that Tat-P promoted autophagy and reduced apoptosis in lung tissues and ameliorated BPD-related phenotypes. Our findings elucidate the underlying mechanisms and effects of Tat-P in enhancing autophagy and preventing apoptosis. This study presents an approach for the prevention and treatment of BPD.

Details

Language :
English
ISSN :
25890042
Volume :
26
Issue :
9
Database :
Directory of Open Access Journals
Journal :
iScience
Publication Type :
Academic Journal
Accession number :
edsdoj.37f95ddce9d14c3881ea5aa430f9455f
Document Type :
article
Full Text :
https://doi.org/10.1016/j.isci.2023.107509