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Whole genome deep sequencing analysis of viral quasispecies diversity and evolution in HBeAg seroconverters
- Source :
- JHEP Reports, Vol 3, Iss 3, Pp 100254- (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Summary: Background & Aims: We aimed to investigate how viral quasispecies of the HBV whole genome evolves and diversifies in response to HBeAg seroconversion and viral control utilising next-generation sequencing (NGS). Methods: Fifty HBeAg-positive chronic hepatitis B patients, including 18 treatment-naïve and 32 interferon (IFN)-treated individuals, were recruited. Serial HBV whole genomes in serum were analysed by NGS to determine sequence characteristics and viral quasispecies. Results: HBV quasispecies diversity, measured by nucleotide diversity, was negatively correlated with viral load and hepatitis activity. Spontaneous HBeAg seroconverters exhibited significantly greater viral quasispecies diversity than treatment-naïve non-seroconverters from >1 year before seroconversion (0.0112 vs. 0.0060, p 1 year after seroconversion (0.0103 vs. 0.0068, p
Details
- Language :
- English
- ISSN :
- 25895559
- Volume :
- 3
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- JHEP Reports
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.38f959d48704517bc685f16fdabf609
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.jhepr.2021.100254