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LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223
- Source :
- Jichu yixue yu linchuang, Vol 43, Iss 1, Pp 102-109 (2023)
- Publication Year :
- 2023
- Publisher :
- Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2023.
-
Abstract
- Objective To investigate the potential effects of long non-coding RNA (lncRNA) MINCR on lipopolysaccharide (LPS)-induced human alveolar epithelial cell line A549 damage and inflammation through targeted regulation of miR-223. Methods A549 cells were randomly divided into control group, LPS group, LPS+transfection group(Si NC group, Si MINCR group, miR-223 NC group, miR-223 mimic group, Si NC+miR-223 NC group, Si NC+miR-223 antagomir group, Si MINCR+miR-223 NC group, and Si MINCR+miR-223 antagomir group). RT-qPCR was performed to determine the expression levels of MINCR and miR-223; Flow cytometry was performed to determine the rate of apoptosis; ELISA was performed to determine the levels of serum tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6); Bioinformatics prediction and dual luciferase reporter gene assay were performed to verify the targeting relationship of MINCR and miR-223; Western blot was performed to test the expression levels of activated caspase-3 (cleaved caspase-3) and B-cell lymphoma-2(Bcl-2) proteins in the cells. Results There was a targeting relationship between MINCR and miR-223. Compared with the control group, the apoptosis rate, the level of TNF-α and IL-6, and the expression of MINCR, cleaved caspase-3 proteins in the LPS group were significantly increased (PPPPPPPConclusions Silencing the expression of lncRNA MINCR may target the activation of miR-223 expression, inhibit cell apoptosis and inflammation, and reduce LPS-induced damage of alveolar epithelial cells.
- Subjects :
- alveolar epithelial cells
lncrna mincr
mir-223
inflammation
cell damage
Medicine
Subjects
Details
- Language :
- Chinese
- ISSN :
- 10016325
- Volume :
- 43
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Jichu yixue yu linchuang
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.3ababa5a77af462fb5b3cc721826716b
- Document Type :
- article
- Full Text :
- https://doi.org/10.16352/j.issn.1001-6325.2023.01.0102