Back to Search Start Over

Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease

Authors :
Wang Chunqiong
Zhu Dan
Sun Haowei
Meng Hongming
Cai Jieyun
Zhang Ke
Zhang Xiaowei
Long Jie
Liu Kai
Chen Dan
Source :
BIO Web of Conferences, Vol 142, p 03010 (2024)
Publication Year :
2024
Publisher :
EDP Sciences, 2024.

Abstract

Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%.

Details

Language :
English, French
ISSN :
21174458
Volume :
142
Database :
Directory of Open Access Journals
Journal :
BIO Web of Conferences
Publication Type :
Academic Journal
Accession number :
edsdoj.3bb00c1adbb49c2894ff31350b9fb9d
Document Type :
article
Full Text :
https://doi.org/10.1051/bioconf/202414203010