Back to Search
Start Over
Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease
- Source :
- BIO Web of Conferences, Vol 142, p 03010 (2024)
- Publication Year :
- 2024
- Publisher :
- EDP Sciences, 2024.
-
Abstract
- Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%.
- Subjects :
- Microbiology
QR1-502
Physiology
QP1-981
Zoology
QL1-991
Subjects
Details
- Language :
- English, French
- ISSN :
- 21174458
- Volume :
- 142
- Database :
- Directory of Open Access Journals
- Journal :
- BIO Web of Conferences
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.3bb00c1adbb49c2894ff31350b9fb9d
- Document Type :
- article
- Full Text :
- https://doi.org/10.1051/bioconf/202414203010