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Protocol for generating high-fidelity proteomic profiles using DROPPS
- Source :
- STAR Protocols, Vol 5, Iss 4, Pp 103397- (2024)
- Publication Year :
- 2024
- Publisher :
- Elsevier, 2024.
-
Abstract
- Summary: Deep mass spectrometry-based proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present a protocol for droplet-based one-pot preparation for proteomic samples (DROPPS), an accessible low-input platform that generates high-fidelity proteomic profiles of 100–2,500 cells. We describe steps for depositing cellular material, cell lysis, and digesting proteins in the same microliter-droplet well. We anticipate DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations.For complete details on the use and execution of this protocol, please refer to Waas et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
- Subjects :
- cell biology
flow cytometry
proteomics
Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 5
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.3be3950dfe8425d9ed66058ed930f65
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2024.103397