Back to Search
Start Over
Sirtuin 3 deficiency promotes acute kidney injury induced by sepsis via mitochondrial dysfunction and apoptosis
- Source :
- Iranian Journal of Basic Medical Sciences, Vol 24, Iss 5, Pp 675-681 (2021)
- Publication Year :
- 2021
- Publisher :
- Mashhad University of Medical Sciences, 2021.
-
Abstract
- Objective(s): To explore the regulation mechanism of Sirtuin 3 (SIRT3) on the mitochondrial function and apoptosis of acute kidney injury (AKI) in septic mice.Materials and Methods: The sepsis-induced AKI model was constructed in the wild-type and SIRT3 knockout (KO) mice, and the levels of serum creatinine (Scr) and plasma kidney injury molecule 1 (pKIM-1) in mice were detected by ELISA. The mitochondrial damage of kidney tubular epithelial cells (KTEC) was observed by electron microscopy, the apoptosis of KTEC was detected by TUNEL assay, and the mRNA levels of SIRT3, Bax, Caspase-3, and Bcl-2 were detected by RT-qPCR.Results: SIRT3 KO caused increased expression of Scr, pKIM-1, and inducible nitric oxide synthase protein in the kidneys of septic mice, and decreased the levels of superoxide dismutase, catalase, and mitochondrial complex enzymes I/II/III/IV. SIRT3 deficiency exacerbated histopathological and mitochondrial damage to the proximal tubules of the kidney. In addition, SIRT3 KO resulted in a significantly increased apoptosis of KTEC, increased the mRNA levels of Bax and Caspase-3, and decreased the mRNA levels of Bcl-2. Conclusion: Our study suggests that SIRT3 deficiency promotes sepsis-induced AKI via increasing oxidative stress, mitochondrial dysfunction, and inducing apoptosis.
- Subjects :
- acute kidney injury
apoptosis
mitochondria
sepsis
sirtuin3
Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 20083866 and 20083874
- Volume :
- 24
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- Iranian Journal of Basic Medical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.3dfd23f390cd48f1b5bcd14302f6faa8
- Document Type :
- article
- Full Text :
- https://doi.org/10.22038/ijbms.2021.54905.12312