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Identification and Structural Analysis of Spirostanol Saponin from Yucca schidigera by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis

Authors :
Jingya Ruan
Lu Qu
Wei Zhao
Chang Gao
Peijian Huang
Dandan Zheng
Lifeng Han
Haiyang Yu
Zixin Zhang
Yi Zhang
Tao Wang
Source :
Molecules, Vol 25, Iss 17, p 3848 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Yucca schidigera Roezl (Mojave), a kind of ornamental plant belonging to the Yucca genus (Agavaceae), whose extract exhibits important roles in food, beverage, cosmetic and feed additives owing to its rich spirostanol saponins. To provide a comprehensive chemical profiling of the spirostanol saponins in it, this study was performed by using a multi-phase liquid chromatography method combining a reversed phase chromatography T3 column with a normal phase chromatography silica column for the separation and an ESI-Q-Exactive-Orbitrap MS in positive ion mode as the detector. By comparing the retention time and ion fragments with standards, thirty-one spirostanol saponins were identified. In addition, according to the summary of the chromatographic retention behaviors and the MS/MS cleavage patterns and biosynthetic pathway, another seventy-nine spirostanol saponins were speculatively identified, forty ones of which were potentially new ones. Moreover, ten novel spirostanol saponins (three pairs of (25R/S)-spirostanol saponin isomer mixtures) were targeted for isolation to verify the speculation. Then, the comprehensive chemical profiling of spirostanol saponins from Y. schidigera was reported here firstly.

Details

Language :
English
ISSN :
14203049
Volume :
25
Issue :
17
Database :
Directory of Open Access Journals
Journal :
Molecules
Publication Type :
Academic Journal
Accession number :
edsdoj.3e45dc21b81643b3b320a17e9385407c
Document Type :
article
Full Text :
https://doi.org/10.3390/molecules25173848