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GhSWEET42 Regulates Flowering Time under Long-Day Conditions in Arabidopsis thaliana

Authors :
Mengxue Du
Deying Wang
Jingyu Li
Taotao Zhu
Peng Lyu
Gang Li
Yi Ding
Xinxin Liu
Qingmei Men
Xiaofei Li
Yongwang Sun
Lingzhi Meng
Shangjing Guo
Source :
Plants, Vol 13, Iss 16, p 2181 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Flowering in plants is pivotal for initiating and advancing reproductive processes, impacting regional adaptation and crop yield. Despite numerous cloned and identified flowering time genes, research in cotton remains sparse. This study identified GhSWEET42 as a key determinant of the flowering time in cotton, demonstrating that its heterologous expression in Arabidopsis accelerated flowering under LD conditions compared to WT. Transgenic plants exhibited upregulated expression of the flowering inducers AtFT, AtSOC1, AtGI, and AtFKF1, alongside downregulated expression of the repressors AtTSF, AtFLC, and AtRGL2, correlating with the earlier flowering phenotype. GhSWEET42 showed a constitutive expression pattern, with elevated levels in the leaves, petals, and flower buds, and was notably higher in early-maturing cotton varieties. Subcellular localization assays confirmed GhSWEET42’s presence on the cell membrane. Transcriptome analysis between WT and GhSWEET42-overexpressing Arabidopsis plants revealed 2393 differentially expressed genes (DEGs), spanning 221 biological processes, 93 molecular functions, and 37 cellular components according to Gene Ontology (GO) enrichment analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis categorized the DEGs into metabolism and environmental information processing. These findings enhance the understanding of GhSWEET42’s function and provide a foundation for elucidating the molecular mechanisms governing flowering time regulation in cotton.

Details

Language :
English
ISSN :
22237747
Volume :
13
Issue :
16
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.402b6878a3ee4e0a99774202730744cd
Document Type :
article
Full Text :
https://doi.org/10.3390/plants13162181