Protective Effect and Its Mechanism Analysis of Baihe Wuyao Decoction on Treatment of Type 1 Diabetes Mellitus and Associated Hepatic Injury

Objective: To investigate the protective effect of Baihe Wuyao decoction (BWD) on mice with type 1 diabetes mellitus (T1DM) and liver damage, and to reveal its underlying mechanism. Methods: Ten of 70 mice were randomly selected as the blank group, and the rest were induced with intra-peritoneally (ip) streptozotocin (STZ) to induce T1DM. After successful modeling, the mice were randomly divided into 6 groups: Model group, positive control group and BWD groups (15, 5, 2.5, 1.25 g·kg−1·d−1). The mice in each group were given continuous administration for 6 weeks. The contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum of mice were measured, and the contents of superoxidedismutase (SOD) and malonaldehyde (MDA) in liver tissue homogenate were detected. The pathological changes of mouse liver were observed. The expression of Protein kinase B (AKT) in mouse liver was detected, the mRNA level of manganese superoxide dismutase (Mn-SOD) and nitric oxide synthase (iNOS), inflammatory factors including tumor necrosis factor (TNF-α), nuclear factor-κB (NF-κB), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), apoptosis factors including B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), Caspase 3 (CASP3) were measured. Results: BWD exhibited a good hepatic protection with ameliorating hepatic pathological morphology in T1DM mice. Compared with the model group, the levels of ALT and AST in each dose group were significantly reduced (P