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Strain-specific differences in the development of bone loss and incidence of osteonecrosis following glucocorticoid treatment in two different mouse strains

Authors :
Kie Shidara
Geetha Mohan
Yan-An Evan Lay
Karl J. Jepsen
Wei Yao
Nancy E. Lane
Source :
Journal of Orthopaedic Translation, Vol 16, Iss , Pp 91-101 (2019)
Publication Year :
2019
Publisher :
Elsevier, 2019.

Abstract

Objective: Glucocorticoids (GCs) are commonly prescribed as treatment for chronic inflammatory diseases. Prolonged use of GCs is a common cause of atraumatic osteonecrosis (ON) and secondary osteoporosis. Currently, there is no effective treatment for this disease; therefore, a reliable animal model would be useful to study both the pathology and novel treatment strategies for patients with the disease. The aim of this study was to establish a validated, reproducible model of GC-induced ON and bone loss in two different mouse strains (BALB/c and C57BL/6). Methods: Seven-week-old male BALB/c (n = 32) and male C57BL/6 mice (n = 32) were randomised into placebo or GC groups and treated with daily 4 mg/L oral dexamethasone in drinking water for 90 days. Study outcome measures included histologic assessment of ON of the distal femur, bone mass and mechanical strength of tibia and lumbar vertebral body, osteoclast number, biochemical measure of bone formation and bone marrow fat quantitation. Results: GC-induced ON lesions were observed in the distal femur in 47% of the male BALB/c mice and 25% of the male C57BL/6 mice. GC treatment decreased the trabecular bone volume and serum pro-collagen type 1N-protease (P1NP) in BALB/c mice compared with the placebo (p

Details

Language :
English
ISSN :
2214031X
Volume :
16
Issue :
91-101
Database :
Directory of Open Access Journals
Journal :
Journal of Orthopaedic Translation
Publication Type :
Academic Journal
Accession number :
edsdoj.40f471e4644849e9a6170ea4f0ea74ce
Document Type :
article
Full Text :
https://doi.org/10.1016/j.jot.2018.07.001