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Measurement of Dilational Modulus of an Adsorbed BSA Film Using Pendant Bubble Tensiometry: From a Clean Interface to Saturation

Authors :
Siam Hussain
Johann Eduardo Maradiaga Rivas
Wen-Chi Tseng
Ruey-Yug Tsay
Boris Noskov
Giuseppe Loglio
Shi-Yow Lin
Source :
Colloids and Interfaces, Vol 8, Iss 1, p 4 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Two open issues on the measurement of the dilational modulus (E) for an adsorbed protein film during the adsorption process have been unacknowledged: how E varies during the adsorption and the length of time needed to attain a stable E value. A new approach for detecting the E variation from a clean air–water interface to saturated film and estimating the time needed to reach a saturated state was proposed. A pendant bubble tensiometer was utilized for measuring the relaxations of surface tension (ST) and surface area (SA), and the E was evaluated from the relaxation data of minute distinct perturbances. The data showed a clear variation in E during the BSA adsorption: E sharply decreased to a minimum at the early stage of BSA adsorption; then, it rose from this minimum and oscillated for a while before reaching an E corresponding to a saturated BSA film after a significant duration. The adsorbed BSA film took ~35 h to reach its saturated state, which was much longer than the reported lifetime of the adsorbed film in the literature. A rapid surface perturbation (forced bubble expansion/compression) could change the E, causing a significant drop in E followed by a slow increase to the original stable value.

Details

Language :
English
ISSN :
25045377
Volume :
8
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Colloids and Interfaces
Publication Type :
Academic Journal
Accession number :
edsdoj.4144243744094f0398cb9b55a3fe51bc
Document Type :
article
Full Text :
https://doi.org/10.3390/colloids8010004