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6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress
- Source :
- Animals, Vol 13, Iss 8, p 1315 (2023)
- Publication Year :
- 2023
- Publisher :
- MDPI AG, 2023.
-
Abstract
- 6-Gingerol, the main active ingredient in ginger, exhibits a variety of biological activities, such as antioxidant, anti-inflammatory, and anticancer activities, and can affect cell development. However, the effects of 6-gingerol on mammalian reproductive processes, especially early embryonic development, are unclear. This study explored whether 6-gingerol can be used to improve the quality of in vitro-cultured porcine embryos. The results showed that 5 μM 6-gingerol significantly increased the blastocyst formation rates of porcine early embryos. 6-Gingerol attenuated intracellular reactive oxygen species accumulation and autophagy, increased intracellular glutathione levels, and increased mitochondrial activity. In addition, 6-gingerol upregulated NANOG, SRY-box transcription factor 2, cytochrome c oxidase subunit II, mechanistic target of rapamycin kinase, and RPTOR independent companion of MTOR complex 2 while downregulating Caspase 3, baculoviral IAP repeat containing 5, autophagy related 12, and Beclin 1. Most importantly, 6-gingerol significantly increased the levels of p-extracellular regulated protein kinase 1/2 while reducing the levels of p-c-Jun N-terminal kinase 1/2/3 and p-p38. These results indicate that 6-gingerol can promote the development of porcine early embryos in vitro.
Details
- Language :
- English
- ISSN :
- 20762615
- Volume :
- 13
- Issue :
- 8
- Database :
- Directory of Open Access Journals
- Journal :
- Animals
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4148a866f9ec476e9055a12a439d2f04
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/ani13081315