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A Novel High-Throughput and Sensitive Electrochemiluminescence Immunoassay System

Authors :
Xiancheng Liu
Shuang Zhao
Yan Xu
Bo Zhang
Junli Huang
Feng Liu
Ning Yang
Wenhua Lu
Dandan Shi
Dezhong Xie
Yuanfang Hou
Guixue Wang
Source :
Bioengineering, Vol 11, Iss 9, p 885 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

(1) Background: In vitro diagnostic (IVD) tests are the main means of obtaining diagnostic information for clinical purposes. The electrochemiluminescence immunoassay (ECLIA) has become an important in vitro diagnostic technique. It has unique advantages and broad market prospects due to its sensitivity, detection limit, detection range and reagent stability. At present, there is a need to develop and optimize electrochemiluminescence immunoassay subsystems to achieve high-throughput outputs and accurate detection of instruments to promote their clinical application. (2) Methods: On the basis of the demand for clinical testing instruments with high detection accuracy and speed, this study constructed an electrochemiluminescence immunoassay system by designing magnetic separation modules, introducing PMT and optimizing the system timing regulation capability. (3) Results: The magnetic separation modules can increase the detection accuracy, the PMT system increases the detection sensitivity and optimized system timing can achieve maximum test output. Furthermore, this system performs well in terms of its linearity, detection limit, signal-to-noise ratio, precision and accuracy. (4) Conclusions: The electrochemiluminescence immunoassay system is capable of high throughput with good sensitivity and accuracy, meeting the basic requirements of clinical applications for detection capacity and output throughput.

Details

Language :
English
ISSN :
23065354
Volume :
11
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Bioengineering
Publication Type :
Academic Journal
Accession number :
edsdoj.420491add4d34e97ad0a6ac545814cf4
Document Type :
article
Full Text :
https://doi.org/10.3390/bioengineering11090885