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Mild hypothermia promotes neuronal differentiation of human neural stem cells via RBM3-SOX11 signaling pathway

Authors :
Yuxiao Ma
Zhenghui He
Jiangchang Wang
Ping Zheng
Zixuan Ma
Qian Liang
Qiao Zhang
Xiongfei Zhao
Jialin Huang
Weiji Weng
Jiyao Jiang
Junfeng Feng
Source :
iScience, Vol 27, Iss 4, Pp 109435- (2024)
Publication Year :
2024
Publisher :
Elsevier, 2024.

Abstract

Summary: Both therapeutic hypothermia and neural stem cells (NSCs) transplantation have shown promise in neuroprotection and neural repair after brain injury. However, the effects of therapeutic hypothermia on neuronal differentiation of NSCs are not elucidated. In this study, we aimed to investigate whether mild hypothermia promoted neuronal differentiation in cultured and transplanted human NSCs (hNSCs). A significant increase in neuronal differentiation rate of hNSCs was found when exposed to 35°C, from 33% to 45% in vitro and from 7% to 15% in vivo. Additionally, single-cell RNA sequencing identified upregulation of RNA-binding motif protein 3 (RBM3) in neuroblast at 35°C, which stabilized the SRY-box transcription factor 11 (SOX11) mRNA and increased its protein expression, leading to an increase in neuronal differentiation of hNSCs. In conclusion, our study highlights that mild hypothermia at 35°C enhances hNSCs-induced neurogenesis through the novel RBM3-SOX11 signaling pathway, and provides a potential treatment strategy in brain disorders.

Details

Language :
English
ISSN :
25890042
Volume :
27
Issue :
4
Database :
Directory of Open Access Journals
Journal :
iScience
Publication Type :
Academic Journal
Accession number :
edsdoj.422d413042e5426687625d1f8fdd0eb8
Document Type :
article
Full Text :
https://doi.org/10.1016/j.isci.2024.109435