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Metabolite differences between glutamate carboxypeptidase II gene knockout mice and their wild-type littermates after traumatic brain injury: a 7-tesla 1H-MRS study

Authors :
Wenbo Wu
Siyi Xu
Jialin Wang
Kuiming Zhang
Mingkun Zhang
Yang Cao
Hongqing Ren
Deyou Zheng
Chunlong Zhong
Source :
BMC Neuroscience, Vol 19, Iss 1, Pp 1-9 (2018)
Publication Year :
2018
Publisher :
BMC, 2018.

Abstract

Abstract Background Traumatic brain injury (TBI) is a complex condition and remains a prominent public and medical health issue in individuals of all ages. A rapid increase in extracellular glutamate occurs after TBI, leading to glutamate-induced excitotoxicity, which causes neuronal damage and further functional impairments. Although inhibition of glutamate carboxypeptidase II (GCP II) is considered a potential approach for reducing glutamate-induced excitotoxicity after TBI, further detailed evidence regarding its efficacy is required. Therefore, in this study, we examined the differences in the metabolite status between wild-type (WT) and GCP II gene-knockout (KO) mice after TBI using proton magnetic resonance spectroscopy (1H-MRS) and T2-weighted magnetic resonance (MR) imaging with a 7-tesla imaging system, and brain water-content analysis. Results Evaluation of glutamate and N-acetylaspartate concentrations revealed a decrease in both levels in the ipsilateral hippocampus at 24 h post-TBI; however, the reduction in glutamate and N-acetylaspartate levels was less marked in GCP II-KO mice than in WT mice (p

Details

Language :
English
ISSN :
14712202
Volume :
19
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Neuroscience
Publication Type :
Academic Journal
Accession number :
edsdoj.42392672260141119ef0c4c624ca2942
Document Type :
article
Full Text :
https://doi.org/10.1186/s12868-018-0473-5