Back to Search
Start Over
Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells
- Source :
- Zhongliu Fangzhi Yanjiu, Vol 50, Iss 11, Pp 1066-1074 (2023)
- Publication Year :
- 2023
- Publisher :
- Magazine House of Cancer Research on Prevention and Treatment, 2023.
-
Abstract
- Objective To explore the effect of KCNQ1OT1 gene knockout combined with bruceine D on the proliferation, migration, and invasion of breast cancer MDA-MB-231 cells. Methods Cell Counting Kit-8, wound healing, and Transwell invasion assay were used to detect the effects of bruceine D and siKCNQ1OT1 on the viability, migration, and invasion of MDA-MB-231 cells. Effect of bruceine D and siKCNQ1OT1 on the expression of KCNQ1OT1 in MDA-MB-231 cells was detected by qRT-PCR. Western blot was used to detect the effect of bruceine D and siKCNQ1OT1 on the expression of EMT-related proteins and CDC42, p-MKK7, MKK7 proteins in MDA-MB-231 cells. Results Bruceine D and siKCNQ1OT1 could significantly inhibit the viability, migration, and invasion of MDA-MB-231 cells, and the inhibitory effect was enhanced when they were combined (all P < 0.05); bruceine D downregulated the expression of KCNQ1OT1 in MDA-MB-231 cells (all P < 0.05); bruceine D combined with siKCNQ1OT1 significantly decreased CDC42, p-MKK7, N-cadherin, and Vimentin expression in MDA-MB-231 cells and increased the expression of E-cadherin (all P < 0.05). Conclusion Bruceine D combined with siKCNQ1OT1 significantly inhibit the proliferation, migration, invasion, and EMT of human breast cancer MDA-MB-231 cells, and its molecular mechanism may be related to the blocking of CDC42/MKK7 signaling pathway.
Details
- Language :
- Chinese
- ISSN :
- 10008578
- Volume :
- 50
- Issue :
- 11
- Database :
- Directory of Open Access Journals
- Journal :
- Zhongliu Fangzhi Yanjiu
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.430bd53726b446dd94bec66b34f61b92
- Document Type :
- article
- Full Text :
- https://doi.org/10.3971/j.issn.1000-8578.2023.23.0456