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Label-free detection and quantification of ultrafine particulate matter in lung and heart of mouse and evaluation of tissue injury

Authors :
Saira Hameed
Kun Pan
Wenhua Su
Miles Trupp
Lan Mi
Jinzhuo Zhao
Source :
Particle and Fibre Toxicology, Vol 19, Iss 1, Pp 1-12 (2022)
Publication Year :
2022
Publisher :
BMC, 2022.

Abstract

Abstract While it is known that air borne ultrafine particulate matter (PM) may pass through the pulmonary circulation of blood at the alveolar level between lung and heart and cross the air-blood barrier, the mechanism and effects are not completely clear. In this study the imaging method fluorescence lifetime imaging microscopy is adopted for visualization with high spatial resolution and quantification of ultrafine PM particles in mouse lung and heart tissues. The results showed that the median numbers of particles in lung of mice exposed to ultrafine particulate matter of diameter less than 2.5 µm was about 2.0 times more than that in the filtered air (FA)-treated mice, and about 1.3 times more in heart of ultrafine PM-treated mice than in FA-treated mice. Interestingly, ultrafine PM particles were more abundant in heart than lung, likely due to how ultrafine PM particles are cleared by phagocytosis and transport via circulation from lungs. Moreover, heart tissues showed inflammation and amyloid deposition. The component analysis of concentrated airborne ultrafine PM particles suggested traffic exhausts and industrial emissions as predominant sources. Our results suggest association of ultrafine PM exposure to chronic lung and heart tissue injuries. The current study supports the contention that industrial air pollution is one of the causative factors for rising levels of chronic pulmonary and cardiac diseases.

Details

Language :
English
ISSN :
17438977
Volume :
19
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Particle and Fibre Toxicology
Publication Type :
Academic Journal
Accession number :
edsdoj.44edb95a90d4e8dbc1258d69e331b41
Document Type :
article
Full Text :
https://doi.org/10.1186/s12989-022-00493-8