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Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan

Authors :
Reiko Suwa
Yohei Kume
Miyuki Kawase
Mina Chishiki
Takashi Ono
Sakurako Norito
Ko Sato
Michiko Okamoto
Satoru Kumaki
Yukio Nagai
Mitsuaki Hosoya
Makoto Takeda
Hidekazu Nishimura
Koichi Hashimoto
Kazuya Shirato
Source :
Pathogens, Vol 11, Iss 7, p 754 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

The World Health Organization initiated a global surveillance system for respiratory syncytial virus (RSV) in 2015, and the pilot surveillance is ongoing. The real-time RT-PCR RSV assays (Pan-RSV and duplex assays) developed by the United States Centers for Disease Control and Prevention are applied as the standard assays. To introduce these as standard assays in Japan, their practicality was evaluated using 2261 specimens obtained from pediatric inpatients in Japan, which were collected from 2018 to 2021. Although the Pan-RSV and duplex assays had similar analytical sensitivities, they yielded 630 (27.9%) and 786 (34.8%) RSV-positive specimens, respectively (p < 0.001). Although sequencing analysis showed mismatches in the reverse primer used in the Pan-RSV assay, these mismatches did not affect its analytical sensitivity. The analysis of read numbers of RSV isolates from air–liquid interface culture of human bronchial/tracheal epithelial cells showed that the duplex assay had a greater number of reads than did the Pan-RSV assay. Therefore, the duplex assay has superior detection performance compared with the Pan-RSV assay, but the two assays have similar analytical sensitivities.

Details

Language :
English
ISSN :
20760817
Volume :
11
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Pathogens
Publication Type :
Academic Journal
Accession number :
edsdoj.4659a3f35ad94046be59ff3d9bc1cdd8
Document Type :
article
Full Text :
https://doi.org/10.3390/pathogens11070754