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3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy
- Source :
- Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
- Publication Year :
- 2022
- Publisher :
- Nature Portfolio, 2022.
-
Abstract
- An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
- Subjects :
- Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 23993642
- Volume :
- 5
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Communications Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.482466fc9d14136bc40195885614206
- Document Type :
- article
- Full Text :
- https://doi.org/10.1038/s42003-022-03390-0