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Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes
- Source :
- Chinese Medicine, Vol 6, Iss 1, p 12 (2011)
- Publication Year :
- 2011
- Publisher :
- BMC, 2011.
-
Abstract
- Abstract Background Arctium lappa (Niubang), a Chinese herbal medicine, is used to treat tissue inflammation. This study investigates the effects of arctigenin (AC), isolated from A. lappa, on anti-CD3/CD28 Ab-stimulated cell proliferation and cytokine gene expression in primary human T lymphocytes. Methods Cell proliferation was determined with enzyme immunoassays and the tritiated thymidine uptake method. Cytokine production and gene expression were analyzed with reverse transcription-polymerase chain reaction. Results AC inhibited primary human T lymphocytes proliferation activated by anti-CD3/CD28 Ab. Cell viability test indicated that the inhibitory effects of AC on primary human T lymphocyte proliferation were not due to direct cytotoxicity. AC suppressed interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in a concentration-dependent manner. Furthermore, AC decreased the IL-2 and IFN-γ gene expression in primary human T lymphocytes induced by anti-CD3/CD28 Ab. Reporter gene analyses revealed that AC decreased NF-AT-mediated reporter gene expression. Conclusion AC inhibited T lymphocyte proliferation and decreased the gene expression of IL-2, IFN-γ and NF-AT.
- Subjects :
- Other systems of medicine
RZ201-999
Subjects
Details
- Language :
- English
- ISSN :
- 17498546
- Volume :
- 6
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4b47fd2417d544698a29a5a88d08cca9
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/1749-8546-6-12